Regeneration Globes are globes that drop when minions and mercenaries are killed; for minion waves, they drop from the ranged wizard. When picked up, they help in replenishing a portion of a hero's health and mana (if they use it).
Globes only last for a short amount of time (six seconds) before disappearing.
Globes that are greyed out are available to enemy players, so prohibiting them from gathering them is part of a good strategy. Purple globes can be picked up by either team and appear when specific creatures are killed on select maps, such as Haunted Mines and Garden of Terror.